Congo red stain control tissue

Components box contains 24 unstained slides plus one reference slide stained using amyloid stain, congo red procedure no. It is used for the detection of amyloid on formalinfixed, paraffinembedded tissue sections with amyloidosis, and may be used for frozen sections as well. Protect paraffin block andor slides from excessive heat. Amyloid is homogeneous and eosinophilic, the deposits are extracellular and may become sufficiently large enough to cause damage to surrounding tissues. Amyloid histology control slides only from american. Congo red stain is used for the visual detection of amyloid in muscle and nerve fresh frozen sections in patients who have amyloidosis. The amyloid fibril congo red complex demonstrates apple green birefringence using polarized light microscopy. This amyloid, puchtler congo red stain kit procedure, is used for the demonstration of amyloid in tissues. The reason for this is that the congo red dye used in the stain is highly selective for amyloid deposits, which can lead to amyloidosis and also alzheimers.

Differentiate in 80% alcohol until excess congo red is removed8. Carnoys and absolute alcohol are recommended, 10% nbf or bouins. Congo red stain protocol for amyloid run a control slide. The main special stain used for amyloid detection is congo red. Transport tissue block or 2 unstained 3 to 5micron thick sections, positively charged slides in a tissue transport kit. Congo red stain is the gold standard for the demonstration of amyloid in tissue sections. Systemic amyloidosis is a devastating group of disorders for which there is no current cure. Special sectioning protocols for various tissue types and. Available online through esupply using arup connect or contact arup client services at 800 5222787.

Revalidate after one year to verify continued reactivity. The amyloid stain kit congo red is intended for use in the histological visualization of amyloid in tissue sections. A, congo redpositive core of alzheimer disease plaque. Bennhold congo red quality control stained slide s included. To demonstrate amyloid deposits in tissue sections. Cresyl violet this histology stain is a nissl stain. Since then this birefringence has been used as a diagnostic for amyloid fibrils. It is used to evaluate the presence and extent of amyloidosis in different organs. Amyloid histology control slides are guaranteed to be the tissue stated, or, are guaranteed to be positive for the organism, structure, or pathology stated. Congo red stain amyloid is a fibrillar protein with a. Congo red stain is used for the visual detection of amyloid in muscle and nerve fresh frozen sections in.

Control slides with reference tissue for the detection of amyloid in histological tissue. In the 1920s benhold and divry established that congo red bound to amyloid in tissue sections and demonstrated its characteristic yellowgreen birefringence under crossed polarizers. Congo red congo red histology stain is used to stain amyloid. Bennhold congo red quality control stained slides included. Modified highmans congo red staining protocol for amyloid. A section of a biopsy with abundant amyloid in the muscle is used as a positive control when a new congo red staining solution is prepared. The addition of the alkaline salt solution in the puchtler modification of the bennhold method reduces background birefringence.

Guaranteed product specific reactivity for one year from date of receipt. Histology slide courtesy of education interactive, human histology photo cd. Sirius red sirius red direct red 80 is related to congo red, and the former dye has been proposed to have an advantage as an amyloid stain because it gives a more intense staining reaction, which increases the contrast with adjacent tissue. Congo red thioflavin t oil red o luxol fast blue cresyl echt violet silver stain for neurons. Amyloid stain, congo red, kit is for use in the determi na tion of amyloid in tissue sections. Examination under a polarizing microscope results in green birefringence of amyloid. Improving sensitivity of amyloid detection by congo red.

They are repelled by the negatively charged cytoplasm and gather around the cells, leaving the cells clear and unstained. Both a standard procedure and a microwave pro ce dure are provided. Modified high ph congo red staining protocol for amyloid. Send block containing tissue biopsy fixed in 10% formalin and paraffin embedded please note tissue must be large enough to obtain at least 50 slides with 8 10um sections. An alternative is to use a dye such as nigrosin or congo red, acidic, negatively charged dyes. Histology slide courtesy of corel professional photos histology cd. Histologistics suggested control tissues special stains. This product should be interpreted by a qualified pathologist in conjunction with histological examination, relevant clinical information, and. Pour off stain and flood slide with a saturated aqueous solution of lithium carbonate.

Congo red is still the standard stain used in most histopathology laboratories to. Common diseases for congo red stain include primary amyloidosis, al amyloid seen in plasma cell dyscrasias, aa amyloid associated with inflammatory conditions. Congo red staining kit by ventana medical systems, inc. Cut the unstained slides at appropriate microns according to the stain ordered or. Common diseases for congo red stain include primary amyloidosis, al. Congo red staining kit is intended for use as a qualitative histologic stain to selectively demonstrate amyloid in formalinfixed, paraffinembedded tissue. This stain is used for the visual detection of amyloid in muscle and nerve fresh frozen sections in patients who have amyloidosis.

Stain amyloid in tissue sections with congo red stain kit amyloid stain ab150663. Formalin ammonium bromide gold sublimate sodium thiosulfate. Amyloid deposits will stain red, while nuclei will stain blue. This attachment is achieved by the nonpolar hydrogen bonds formed between the congo red and amyloid deposit. Amyloid, an abnormal protein product of an immuno log ic disturbance, accumulates. Does anyone have a working protocol for preparation of. Amyloidosis is a rare disease characterized by the deposition of insoluble misfolded proteins in various tissues and organs. Amyloid deep red to pink elastic fibers pale pink nuclei blue. The birefringence assay is not a simple one, for example, the tissue sections need to be of a required thickness to show. Vitroview congo red amyloid stain kit vitrovivo biotech. Amyloid, puchtler congo red stain kit newcomer supply.

This technique is called the negative stain technique. Therefore, it is recommended that control sections be relatively freshly cut. The congo red stain kit amyloid stain kit is intended for use in the histological visualization of amyloid in tissue sections. Amyloid tissuetrol control slide has been used as a positive control in amyloid staining of pancreatic sections. Simply compare your preparations with the control tissue specimens to be. This product should be interpreted by a qualified pathologist in conjunction with histological examination, relevant clinical information, and proper controls. Amyloid stain reagents are for in vitro diagnostic use. The treatment goal is to reduce the burden of amyloidogenic protein precursors. This b ennhold s congo red stain is used for the detection of amyloid on formalinfixed, paraffinembedded tissue sections with amyloidosis, and may be used for frozen sections as well. Paraffin sections cut at 8 microns on superfrost plus slides. Congo red is an organic compound used for staining amyloid in tissue sections. The amyloid deposits will be stained red and the nuclei will be stained blue.

Unstained slides are requested when stains are ordered. B, applegreen birefringence of amyloid core under polarized. Congo red amyloid stain kit is used for the detection of amyloid in formalinfixed, paraffinembedded tissue sections with amyloidosis, and may be used for frozen sections as well. The dyeing of amyloid is by a mechanism similar to the direct textile dyeing of cotton. Formalin fix 10 percent neutral buffered formalin is preferred and paraffin embed specimen cells must be prepared into a cellblock. Bielschowsky stain for senile plaques bile stain bodians stain colloidal iron stain congo red stain copper stain elastic van gieson stain elastic weigerts resorcinfuchsin method modified elastic van gieson stain fontanamasson stain for melanin melanin bleach fraser lendrum stain giemsa modified maygruenwald stain for hematopoietic.

Each stain gave similar representative eosinophil numbers, had minimal neutrophil staining and had good contrast between eosinophils and background tissue for a userfriendly readability. When i incorporated congo red dye into the cmc agar 0. Congo red cr is the most popular dye used as a probe for diagnosing amyloidosis, a very heterogeneous group of diseases with more than 23 chemically different amyloid syndromes of men and animals, leading to more than 400 different individual diseases. Overview the amyloid stain congo red is intended for use in the histological visualization of amyloid in tissue sections. The treatment is only effective if applied early in the disease process before significant and irreversible end organ damage has taken place. The congo red stain can be applied to tissues fixed in a variety of fixative solutions. New isoslide afb and congo red control slides for histology.

Congo red, despite having lower sensitivity, is the standard agent used to identify amyloid in tissues. Amyloid animal control histology slides newcomer supply. The congo red stain kit is composed of three readytouse dispenser packs, containing. Congo red stain protocol for amyloid histopathology service. Comparison of histochemical methods for murine eosinophil. Slides can be stained using the mastertech alkaline congo red amyloid stain kit, mastertech congo red amyloid stain kit, mastertech p. Stain kit, or with mastertech crystal violet stain. Suggested control tissues special stains written by hans b snyder acid fast bacilli afb acid fast bacteria alcian blue 1.